TitleComparative and evolutionary analysis in natural diploid and tetraploid weather loach Misgurnus anguillicaudatus based on cytochrome b sequence data in central China
Publication TypeJournal Article
Year of Publication2009
AuthorsYang, C, Cao, L, Wang, WM, Yi, Y, Abbas, K, Yan, B, Wang, H, Su, L, Sun, Y, Wang, H
JournalEnvironmental Biology of Fishes
Volume86
Issue1
Pagination145 - 153
Date Published2009
ISBN Number0378-1909
KeywordsCytochrome b, Evolution, Genetic distance, Genetic variation, Misgurnus anguillicaudatus, Ploidy
Abstract

To obtain the phylogenetic relationship between diploid and tetraploid Misgurnus anguillicaudatus, the mitochondrial cyt b gene in the diploid and tetraploid weather loach were isolated and sequenced. The DNA sequences were analyzed using MEGA 3.0 software to determine the phylogenetic relationship. Forty-five variable sites among cyt b gene sequences and 18 amino acid substitutions occurred within the diploid and tetraploid loaches as deduced from the nucleotide sequences analysis of the cyt b gene. The nucleotide pairwise distance between diploid and tetraploid loach ranged from 0.001 to 0.025. Phylogenetic analysis revealed evolutionary relationships between diploid and tetraploid loach. Our results indicated a significant difference between diploid and tetraploid loach about the cyt b gene. ANOVA analysis indicated that there were no significant genetic variations within diploid loaches (Fst = 0.2529, P > 0.05) and within tetraploid loaches (Fst = 0.0564, P > 0.05), neither. However, significant genetic differences were found between diploid and tetraploid loaches (Fst = 0.7634, P < 0.05). Thus, it is concluded that no reproductive isolation was found within the same cytotypes of different localities, but there was reproductive isolation between these two cytotypes. The diploid loach existed before the tetraploid loach in nature. The present study is the first to describe the phylogenetic relationships of natural polyploidy weather loach using mtDNA cyt b gene.
URLhttps://link.springer.com/article/10.1007/s10641-007-9283-9
Notice of Publication Number

NOP 07-227 (English)