Feed the Future Innovation Lab for Collaborative Research on Aquaculture & Fisheries

Growth, plasma steroids, and the appearance of gonads (histology and gonadosomatic index) were followed in South American catfish (surubim, Pseudoplatystoma sp.) raised in captivity in the aquaculture facility at The Ohio State University, Columbus, Ohio, USA, from 2003 until 2006. Broodstock growth showed a great individual variation and it did not seem sex dependent. The levels of 11-ketotestosterone were high in males during the reproductive season. Three out of six females spawned after receiving two doses of carp pituitary extract (0.5 and 5 mg/kg) at 11-h intervals. Fertilization was performed in only one female in February 2006. Egg size was 0.73 +/- 0.06 mm in diameter at stripping. Two males released sperm, and it was used for fertilization. Sperm concentrations were 24 x 10⁹ and 15.5 x 10⁹ spermatozoa/mL in Male 1 and Male 2, respectively, and viability was confirmed after activation in 0.3% NaCl. Embryo survival at 9 h after fertilization was 44 and 23% for Male 1 and Male 2, respectively. Embryos hatched 15 h after fertilization. Larvae were 3.53 +/- 0.09 mm in length at hatching and were successfully raised (72% survival after 2 wks) using live brine shrimp nauplii.

NOP 08-231 (English)