TitleDevelopment of digestive enzymes in larvae of Mayan cichlid Cichlasoma urophthalmus
Publication TypeJournal Article
Year of Publication2011
AuthorsLopez-Ramirez, G, Cuenca-Soria, CA, Álvarez-González, CA, Tovar-Ramirez, D, Ortiz-Galindo, JL, Perales-Garcia, N, Marquez-Couturier, G, Arias-Rodriguez, L, Indy, JR, Contreras-Sánchez, WM, Gisbert, E, Moyano, FJ
JournalFish Physiology and Biochemistry
Pagination197 - 208
Date Published2011
Keywordsa-Amylase, Cichlasoma urophthalmus, Lipase, Mayan cichlid, Phosphatase, Zymogram

The development of digestive enzymes during the early ontogeny of the Mayan cichlid (Cichlasoma urophthalmus) was studied using bio- chemical and electrophoretic techniques. From yolk absorption (6 days after hatching: dah), larvae were fed Artemia nauplii until 15 dah, afterward they were fed with commercial microparticulated trout food (45% protein and 16% lipids) from 16 to 60 dah. Several samples were collected including yolk-sac larvae (considered as day 1 after hatching) and specimens up to 60 dah. Most digestive enzymes were present from yolk absorption (5–6 dah), except for the specific acid proteases activity (pepsin-like), which increase rapidly from 8 dah up to 20 dah. Three alkaline proteases isoforms (24.0, 24.8, 84.5 kDa) were detected at 8 dah using SDS–PAGE zymogram, corresponding to trypsin, chymotrypsin and probably leucine aminopeptidase enzymes, and only one isoform was detected (relative electromobility, Rf = 0.54) for acid proteases (pepsin-like) from 3 dah onwards using PAGE zymogram. We concluded that C. urophthamus is a precocious fish with a great capacity to digest all kinds of food items, including artificial diets provided from 13 dah.

Notice of Publication Number

NOP 11-270 (English)