TitleDNA extraction from crayfish exoskeleton
Publication TypeJournal Article
Year of Publication2011
AuthorsLi, Y, Wang, WM, Liu, X, Luo, W, Zhang, J, Gul, Y
JournalIndian Journal of Experimental Biology
Pagination953 - 957
Date Published2011
ISBN Number8627872821
KeywordsCrayfish exoskeleton, DNA amplification, DNA extraction

Crayfish exoskeleton (CE) samples are generally less invasive and easy to be collected. However, it is difficult to extract DNA from them. This study was intended to investigate CE as a DNA source and design an easy and efficient DNA extraction protocol for polymerase chain reactions. Specific primer pair (PPO-F, PPO-R) was used to amplify extracted DNA from CE, and compared to crayfish tail muscle DNA sample. Moreover, seven microsatellites markers were used to amplify the CE DNA samples set. Since the extracted DNA from CE is suitable for gene amplification, the results present usefulness of CE as an easy and convenient DNA source for PCR-based population genetic research.

Notice of Publication Number

NOP 11-281 (English)