TitleProduction of "Chame" (Dormitator latifrons, Pisces: Eleotridae) larvae using GnRHa and LHRHa
Publication TypeJournal Article
Year of Publication2012
Authorsde Oca, GARodriguez-, Medina-Hernández, EA, Velázquez-Sandoval, J, López-López, VV, Román-Reyes, JC, Dabrowski, K, Haws, MC
JournalRevista Colombiana de Ciencias Pecuarias
Pagination422 - 429
Date Published2012
KeywordsChame fish, Gamete release, Pacific fat sleeper, Spawning, Spermiation

The Pacific fat sleeper is a potential species for aquaculture in Latin American countries. Nevertheless, production depends on wild caught juveniles, thus needing hatchery produced larvae. Objective: the purpose of this study was to determine the ideal conditions for viable gamete release and larvae laboratory production. Methods: a total of 16 mature male and 16 female fish were allocated to one of four groups (n = 4) that were injected with either saline solution, Desgly10-Ala6 LHRHa, salmon GnRHa + domperidone, or implanted with salmon GnRHa. Results: spermiation was observed in all treatments. Spawning rates were 100% at 24 and 48 h for the GnRHa implanted group, 25% for the LHRHa group, and 0% for the salmon GnRHa + domperidone group (48-72 h post injection). Conclusion: GnRHa and LHRHa are a successful tool for chame induced reproduction. A gross morphological description of oocytes, sperm quality, and first stages of larval development is included.

Notice of Publication Number

NOP 12-308 (English)